基于响应面设计的复方赶黄草颗粒的制剂优化

摘要

采用响应面法(response surface method,RSM)确定复方赶黄草颗粒中两味中药延胡索和虎杖的提取工艺,在保证有效成分延胡索乙素和虎杖苷的提取率前提下,改善颗粒的溶解性和病人的顺应性.在对乙醇浓度、药材与溶剂提取比例、提取时间、提取次数进行单因素试验的基础上,利用Box-Behnken试验设计,得到延胡索乙素和虎杖苷提取率的响应面数学模型,预测并验证了工艺参数.通过RSM优化的方法可以得到延胡索和虎杖的最佳提取工艺,分别为乙醇体积分数83%,药材-溶剂比例1∶9,提取时间2 h.在此条件下延胡索乙素提取率为(7.59±0.72)%,虎杖苷提取率为(5.75±0.78)%,能够满足颗粒剂的制剂需要.结论:响应面设计可以用于中药有效成分的提取工艺优化,为复方赶黄草颗粒的增效减毒和制剂优化提供了科学依据.%The extracting conditions of two compounds in compound Penthori herba granule wereoptimized through response surface method (RSM), and the effect on extraction rate of tetrahydropalmatine from Corydalis yanhusuo and polydatin from Polygonum cuspidatum were analyzed.On the basis of single factor experiment, the concentration of ethanol, the proportion of extraction, extraction time and extraction times was evaluated.Using the Box-Behnken design, the mathematical model of tetrahydropalmatine and polydatin extraction rate was obtained.The optimized extracting conditions are as follows: the ethanol concentration is 83%, the extraction ratio is 1∶9 and the extraction time is 2 h.Under this condition, the extraction rate of tetrahydropalmatine is (7.59±0.72)%, the extraction rate of polydatin is (5.75±0.78)%.The best extraction conditions of tetrahydropalmatine and polydatin have been determined, it is useful to provide scientific basis for the follow-up study of compound Penthori Herba granule.