To understand the uptake and accumulation for different valence inorganic selenium in Danshen plants, Danshen was treated by spraying the leaves with sodium selenite and sodium selenate aqueous solution containing 0.060, 0.120, 0.240 g/L Se respectively.There were three Danshen plants treated at every concentration.When the plants were in full-bloom stage, spraying were performed twice with a 10-day interval.The Se contents in the three different tissues (stem, leaf and root) and the soil were determined by hydride generation atomic fluorescence spectrometry (HG-AFS).The results show that Se content in Danshen plants increases with the increase of selenium fertilizer concentration but there is no significant linearity.The Se content in stem of Danshen plant is the highest, followed by leavf and root.Partial selenium is moved and exchanged to soil by plant and the Se content of soil increases with the increase of dosage of Se fertilize.The content of Se in root of Danshen sprayed with selenate and its corresponding soil are higher compared with Danshen sprayed with selenite.In contrast to the root, Se content in stem and leaf of plants spraying with selenite is higher.It seems that selenate has a better mobility in Danshen than selenite.The Se content of Danshen plants can be effectively increased with spraying both kind of inorganic selenium aqueous solution.The selection of selenium reagent for Se supplement still depends on the results of speciation analysis of selenium.%为了解丹参植株对不同价态无机硒的吸收积累情况,采用田间栽种叶面喷施亚硒酸钠和硒酸钠水溶液的方式,分别设置空白对照组和喷施Se质量浓度为0.060、0.120、0.240 g/L的剂量组,每1种处理重复3次,在丹参盛花期间隔10 d喷施2次.采用氢化物发生-原子荧光法测定丹参植株根、茎、叶和土壤中的硒含量.结果表明,丹参植株硒含量随喷施剂量的增加而增加,但增加量与喷施剂量增加不成正比;硒在丹参植株中的含量为茎最高,叶次之,根最低,部分硒经植株迁移交换到土壤,土壤中硒含量与喷施剂量成正相关;喷施硒酸钠的丹参根及对应土壤中硒含量高于喷施亚硒酸钠的丹参根和土壤,而茎和叶中的含量低于喷施亚硒酸钠的实验组.该现象表明硒酸钠比亚硒酸钠在丹参植株-土壤系统中有更好的迁移性.叶面喷施2种价态的无机硒均能有效增加丹参植株的硒含量,选择何种作为补硒试剂还需结合对植株硒的形态分析结果进行综合考虑.
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