目的 建立同时测定柴辛鼻敏康颗粒中芍药苷和阿魏酸含量的方法.方法 采用反相高效液相色谱法.色谱柱为Shiseido ODS柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1%磷酸溶液(20:80),流速为1.0 mL/min,检测波长为芍药苷230 nm、阿魏酸320 nm,柱温30℃.结果 芍药苷和阿魏酸进样量分别在0.512-15,36μg(r=0.999 8)和0.065-1.95μg(r:0.9999)范围内与峰面积线性关系良好(r=0.9999),平均加样回收率分别为99.87%和100.32%,RSD分别为0.97%和1.17%(,n=6).结论 该方法简单、准确度高、专属性强.可有效控制柴辛鼻敏康颗粒的质量.%Objective To establish a method for the simultaneous determination of paeoniflorin and ferulic acid in Chaixinbiminkang Granules under different UV - visible wavelengths. Methods The RP - HPLC method was used to test the content of paeoniflorin and ferulic acid. The separation column Shiseido ODS columm(250 mm×4. 6 mm, 5 μm) was used with acetonitrile - 0. 1% phosphoric acid (20:80) as the mobile phase. The flow rate was 1. 0 mL/min. The paeoniflorin and ferulic acid were determined by dual wavelength, λ1 = 230 nm, λ2=320nm. The column temperature was 30 ℃. Results The linear range of paeoniflorin was 0.512 - 15.36 μg (r =0. 999 8), the average recovery rate was 99.87%, RSD = 0.97% (n = 6).The linear range of ferulic acid was 0.065-1.95 μg( r = 0. 999 9), the average recovery was 100.32%, RSD = 1. 17% (n = 6). Conclusion The method is precise and simple with strong specificity and effective control on the quality of Chaixinbiminkang Granules.
展开▼
