天麻药材高效液相色谱指纹图谱研究

摘要

目的 研究天麻药材的高效液相色谱指纹图谱,为其质量控制提供可靠的方法.方法 采用高效液相色谱法.色谱柱为Uhimate XB-C18柱(250mm×4.6 mm,5 μm),流动相为甲醇-1%冰醋酸水溶液(梯度洗脱),柱温为30℃,流速1 mL/min,检测波长270 am.结果 初步建立了天麻药材的高效液相色谱指纹图谱,标定出16个共有峰;天麻素的加样加收率为102.66%.RSD<2.5%(/n=6).结论 高效液相色谱指纹图谱为天麻药材质量控制提供了一种新方法,并为未知天麻样品的鉴别提供一个准确、快捷的工具.%Objective To study the HPLC fingerprint of medicinal material Gastrodia elata Blume to provide the reliable method for its quality control. Methods The analysis was performed on Ultimate XB- C18 column (250 mm ×4. 6 mm, 5 μm) with the mobile phase of methanal- 1％ glacial acetic acid aqueous solution in the gradient elution. The column temperature was 30 ℃ and the flow rate was 1. 0 mL/min. The UV detector was set at 270 nm. Results The HPLC fingerprint of Gastrodia elata Blume was preliminarily established and 16 common peaks were displayed in the fingerprint. The average sample recovery rate of gastrodine was 102. 66％, RSD ＜ 2. 5％ ( n =6). Conclusion The HPLC fingerprint may provide a new model for controlling the quality of medicinal material Gastrodia elata Blume and also may provide an accurate and rapid tool for identifying unknown sample of Gastrodia elata Blume.