Objective To obtain the constituents of Gastrodia elata Bl. in rat plasma. Methods The rats were fasted 12 h before administration, and the orally dose was 2 mL/100 g. The plasma samples were pre-treated with C18 solid-phase extraction ( SPE ) method. The constituents in rat plasma were analyzed by HPLC-LTQ-Orbitrap MS. Results A total of 10 constituents were detected. Conclusion In addition to gastrodin, 3, 4-dihydroxybenz aldehyde, p-hydroxybenzaldehyde, dimethyl phthalate, gastrodeoside, 2-ethoxy-4-methylphenol, p-ethoxymethylphenyl-1-O-β-glucopyranoside, 4-hydroxybenzyl-β-D-glucopyranoside, 4, 4'-dihydroxydibenzyl ether and 4, 4'-dihydroxydibenzyl methane are into blood as well, they are phenolic compounds and glycosides.%目的 确证天麻入血成分信息.方法 大鼠禁食12 h,灌胃给药,给药量为2 mL/100 g,固相萃取( SPE )法进行血浆处理,运用HPLC-LTQ-Orbitrap MS法对进入大鼠血液的天麻化学成分进行检测.结果 共检测到10种入血成分.结论 除了天麻苷,天麻中还有对羟基苯甲醛、3,4-二羟基苯甲醛、p-ethoxymethylphenyl-1-O-β-glucopyranoside、羟基苄基-1-O-β- D-吡喃葡萄糖、邻苯二甲酸二甲酯、4,4'-二羟基二苄基醚、4,4'-二羟基二苯基甲烷、天麻醚苷、2-乙氧基-4-甲基苯酚进入血液,均为酚性化合物及其苷类.
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