反相高效液相色谱法测定金嗓口服液中绿原酸含量

摘要

目的：建立测定金嗓口服液中绿原酸含量的反相高效液相色谱（RP-HPLC）法。方法色谱柱为Ultimate ODS C18柱（250 mm ×4.6 mm，5μm），乙腈-0.5%磷酸溶液（26：74）为流动相，检测波长为328 nm，流速为1.0 m/min，进样量为10μL。结果绿原酸质量浓度在0.0105~0.1260 g/L范围内与峰面积呈良好线性关系（ r=0.9998），平均回收率为98.08%，RSD=0.73%（ n=6）。结论该方法准确、简便、专属性强、重复性好，可用于测定金嗓口服液中绿原酸的含量。%Objective To establish a RP-HPLC method for the content determination of chorogenic acid in Jinsang Oral Liq-uid. Methods The determination was performed on the Ultimate ODS C18 column(250 mm × 4. 6 mm,5 μm) with the mobile phase of acetonitrile-0. 5% phosphoric acid(26 :74)at the flow rate of 1. 0 mL/min. The detection wavelength was set at 328 nm and the injection volume was 10 μL. Results The linear range of chorogenic acid was 0. 010 5-1. 260 μg/mL( r=0. 999 8) with an average recovery rate of 98. 08% RSD=0. 73% ( n=6). Conclusion The method is simple,accurate and strongly specific with good repro-ducibility and can be used for the content determination of chorogenic acid in Jinsang Oral Liquid.