清益宁络方对IgA肾病小鼠氧自由基的影响

摘要

目的 探讨清益宁络方对IgA肾病小鼠氧自由基的影响.方法 根据完全随机设计原理将50只小鼠均分为空白组、模型组、雷公藤多苷组[5 mg/(kg·d)]、清益宁络方低剂量组[20g/(kg·d)]及高剂量组[40 g/(kg·d)].采用牛血清白蛋白和葡萄球菌肠毒素B复合感染的方法建立小鼠IgA肾病模型,于造模后第8周起灌胃给予相应药物,空白组及模型组给予等体积蒸馏水,连续5周.比较各组给药前及给药后1、3、5周出现血尿和蛋白尿鼠数差异;给药结束后电镜下观察肾脏病理学改变,检测并比较各组肾脏超氧化物歧化酶(SOD)活性及丙二醛含量.结果 模型组2只小鼠、雷公藤多苷组1只小鼠、清益宁络方低剂量组1只小鼠、清益宁络方高剂量组2只小鼠死亡.用药前和用药后1、3周,模型组、雷公藤多苷组及清益宁络方低、高剂量组出现血尿和蛋白尿鼠数组间比较差异无统计学意义(P＞0.05),用药后5周,雷公藤多苷组及清益宁络方低、高剂量组出现血尿和蛋白尿鼠数均少于模型组,差异有统计学意义[血尿:3、2、3只比8只,蛋白尿:3、2、2只比8只;P＜0.05],清益宁络方低、高剂量组与雷公藤多苷组两两比较差异无统计学意义(P＞0.05).与空白组比较,模型组SOD活性明显低,丙二醛含量明显高[(125±28)kU/L比(169±17) kU/L,(20.2±3.2) μmol/L比(14.8±2.4) μmol/L,P＜0.01];与模型组比较,雷公藤多苷组和清益宁络方低、高剂量组SOD活性明显高,丙二醛含量明显低[(149±30)、(148±16)、(162±38) kU/L,(16.5±2.7)、(16.0±3.7)、(15.7±3.4) μmol/L,P＜0.05或P＜0.01].电镜下见雷公藤多苷组和清益宁络方高、低剂量组肾脏病理学改变均较模型组减轻.结论 清益宁络方可改善IgA肾病小鼠血尿和蛋白尿症状,减轻肾脏损伤,提高肾组织中SOD活性及降低丙二醛含量,其机制可能与抗氧自由基损伤有关.%Objective To investigate the effect of Qingyiningluo decoction (QYNLD) on the oxygen free radicals in mouse model of IgA nephropathy.Methods Fifty mice were randomly divided into blank group,model group,tripterygium glycosides (TG) group [5 mg/(kg · d)],QYNLD low dose group [20 g/(kg · d)] and QYNLD high dose group [40 g/(kg · d)] (each n =10).The IgA nephropathy was induced by using bovine serum albumin combined with staphyloentero-toxin B compound infection;intragastric administrations of different drugs were given on the 8th week after establishment of the model and they were continued for 5 weeks.The numbers of the mice with hematuria and proteinuria before and 1,3,5 weeks after treatment were compared;at the end of treatment,the kidney pathology changes were observed;the superoxide dismutase (SOD) and malonaldehyde (MDA) in the kidney tissuewere measured and compared.Results Overall 2 in model group,1 in TG group,1 in QYNLD low dose group and 2 in QYNLD high dose group died.The numbers of mice with hematuria and proteinuria before and 1,3 weeks after treatment had no significantly differences among different groups.Five weeks after treatment,the numbers of mice with hematuria and proteinuria in TG group,QYNLD low dose group and high dose group were all less than those in model group [hematuria:3,2,3 vs 8,proteinuria:3,2,2 vs 8,all P ＜ 0.05];no statistical differences were found among TG,QYNLD low dose and QYNLD high dose group (P ＞ 0.05).The SOD activity was significantly lower and the MDA level was significantly higher in model group [(125 ±28) kU/L vs (169 ± 17) kU/L,(20.2 ±3.2) μmol/L vs (14.8 ±2.4) μmol/L,P＜0.01] than those in blank group;the SOD activity was significantly increased and the MDA level was significantly reduced in TG,QYNLD low dose and QYNLD high dose group [(149 ± 30),(148 ± 16),(162± 38) kU/L,(16.5 ± 2.7),(16.0 ± 3.7),(15.7 ± 3.4) μmol/L,P ＜ 0.05 or P ＜ 0.01] compared with those in model group.The pathological damages of kidney were improved in TG,QYNLD low dose and QYNLD high dose group compared with those in model group.Conclusion QYNLD can improve the hematuria and proteinuria,reduce the kidney damage,increase the SOD activity and reduce the MDA level in the kidney in IgA nephropathy,which may be related to the function of antioxidant.