小鼠脑脊液镁离子浓度及血清镁离子浓度变化对它的影响

摘要

目的 探讨小鼠正常脑脊液镁离子的浓度及血清镁离子浓度改变后对它的影响.方法 使用戊巴比妥钠(50mg/kg)麻醉动物.在鼠尾尖端1~1.5cm处剪断鼠尾,收集血液;剥离小鼠后颈部皮肤肌肉,暴露硬脑膜,使用蝶形针在枕大池内吸取脑脊液;用感应耦合等离子体质谱仪(ICP-MS)检测C57BL/6型(B6型)和ICR型小鼠正常脑脊液和血清镁离子浓度,并检测B6型小鼠腹腔注射硫酸镁(1.23g/kg) 20min、40min、2h、4h后脑脊液和血清镁离子浓度的改变.结果 正常B6型小鼠脑脊液和血清镁离子浓度分别是(0.89±0.11)mM和(1.38±0.12)mM.正常ICR型小鼠脑脊液和血清镁离子浓度分别是(1.00±0.12)mM和(1.10±0.09)mM.B6型小鼠腹膜内注射硫酸镁后血清镁离子浓度从(1.38±0.12)mM(正常水平)增加到(8.68 ± 1.85)mM(注射20min后,P<0.01)、(7.58±0.67)mM(40min后,P<0.01)、(3.56 ± 0.96) mM(2h后,P<0.01)并在4h后减少到正常水平(F=1.514),而脑脊液镁离子浓度未发生明显改变(F =126.884,P>0.05).结论 小鼠脑脊液镁离子浓度不随血清镁离子浓度升高而改变.%Objective To study the influence of changes of cerebrospinal fluid magnesium concentration and serum magnesium concentration in mice.MethodsTo anaesthetize animal using by pentobarbital sodium (50mg/kg).To shear off the mice tail at 1-1.5cm rattail tip to collect blood,to peel the skin and muscle on mice posterior neck to expose endocranium,to absorb cerebrospinal fluid at cisterna magna used by butterfly needle.To detect the cerebrospinal fluid and serum magnesium concentration of C57BL/6 (B6) and ICR mice by inductively coupled plasma mass spectrometry (ICP-MS),to detect the changes of cerebrospinal fluid and serum magnesium concentration in B6 mice which were treated with intraperitoneal injection magnesium sulphuricum(1.23g/kg) after 20min,40min,2h,4h.ResultsThe cerebrospinal fluid and serum magnesium concentration in B6 mice was (0.89±0.11) mM and (1.38±0.12) mM,while which was (1.00±0.12) mM and (1.10±0.09) mM in ICR mice.The serum magnesium concentration in B6 mice has increased from (1.38±0.12) mM to (8.68±1.85) mM after 20min intraperitoneal injection magnesium sulphuricum(P<0.01),to (7.58±0.67) mM after 40min intraperitoneal injection magnesium sulphuricum(P<0.01),to (3.56±0.96) mM after 2h intraperitoneal injection magnesium sulphuricum(P<0.01),then reduced to normal level after 4h intraperitoneal injection magnesium sulphuricum(F=1.514),while cerebrospinal fluid magnesium concentration hadn't occurrence obviously significant change(F=126.884,P>0.05).Conclusion Cerebrospinal fluid magnesium concentration in mice dosn't has change along with serum magnesium concentration.