骨基质明胶在异位成骨区的成骨研究

摘要

Objective To investigate the osteogenic effect of bone matrix gelatin after different dealing in ectopic bone area femoral. Methods 36 Japanese big ears white rabbits were divided into group A, B and C (12 in each). Group A: bFGF+ Vascular bundle+BMG. Group B: Vascular bundle+BMG. Group C: BMG. The animals were sacrificed after 4, 8 and 12 weeks respectively by perfusing ink. HE and Masson stained for histological observations and quantitative analysis. Results Quantitative analysis showed that the osteogenic and neovascularization areas of group A were the largest on the every time point. The correlation analysis of osteogenic area and vascular area were r4weeks=0.53, P < 0.05; r8weeks=0.46, P < 0.05; r12weeks= 0.61, P< 0.05. Conclusion The better vascularization, the better osteogenesis. There is a positive correlation between os-teogenicarea and vascularareawere%目的 探讨不同处理后的骨基质明胶(BMG)在异位成骨区的成骨效果.方法 将36 只日本大耳白兔随机分为3 组,每组12 只.A 组血管束植入用bFGF 预处理过的BMG 中;B 组血管束植入未作任何预处理的BMG 中;C 组单独植入BMG.分别于术后4、8、12 周给各组动物灌注墨汁后做HE 及Masson 染色进行组织学观察和定量分析.结果 新生骨面积定量分析,各个时间点上A 组新生骨面积均最大,与C 组比较差异均有统计学意义(均P ﹤0.05).新生骨面积比与血管面积比相关分析结果显示,r4 周=0.53,P﹤0.05;r8 周=0.46,P﹤0.05;r12 周=0.61,P﹤0.05.结论 血管化程度越高,新生骨面积越大;新生骨面积比与血管化程度呈正相关.