胎膜早破孕妇血清白细胞介素-6、细胞黏附因子-1的表达及其临床意义

摘要

Objective To study the content and expression of interleukin -6 (TL-6) and intercellular adhesion molecule-1 (TCAM-1) in serum of pregnant women with premature rupture of membranes (PROM) and further to explore their relations with PROM. Methods 80 pregnant women with PROM who were operated by caesarean section delivery in our hospital from January 2010 to June 2012 were selected as observation group, 40 pregnant women operated by selective caesarean section in the same period were categorized into control group. Patients in observation group were divided into mature PROM group and premature PROM group according to the time of rupture of membranes; patients in observation group were divided into PROM uninfected group, PROM mild infection group and PROM moderate infection group according to the chorioamnionitis pathological degree. Levels of II.--6 and ICAM-1 were determined In pregnant women serum by enzyme linked immunosorbent assay (ELISA), fetal membrane was performed by histopatho-logie examination after placenta! expulsion. Results ①IL-6 and IACM-1 in observation group were all higher than those in control group, IL-6 and IACM-1 in premature PROM group were all higher than those in mature PROM group, the differences were all statistically significant fall P ＜ 0.01). ②Levels of IL-6 and ICAM-1 in maternal serum In PROM moderate Infection group were higher than those In PROM uninfected group and PROM mild Infection group, the differences were all statistically significant (all P ＜ 0.01); the differences of IL-6 and IACM-1 between the PROM uninfected group and PROM mild infection group were not statistically significant (P ＞ 0.05). Conclusion Infection Is closely related with PROM. IL-6 and ICAM-1 are good to diagnose subclinical Infection of PROM.%目的 研究白细胞介素-6(IL-6)、细胞黏附因子-1(IACM-1)在胎膜早破(PROM)产妇血清中的含量及表达,探讨其与PROM的关系.方法 选择2010年1月～2012年6月我院产科住院行剖宫产分娩的PROM孕妇80例为观察组,选取同期住院行剖宫产的正常孕妇40例为对照组;观察组按胎膜破裂的时间,再分为足月PROM组与未足月PROM组;按绒毛膜羊膜炎病理程度分为PROM未感染组、PROM轻度感染组和PROM中度感染组.用酶联免疫吸附法(ELISA)测定孕妇血清中IL-6、IACM-1水平;胎盘娩出后取胎膜组织进行病理组织学检查.结果 ①观察组血清IL-6、IACM-1浓度高于对照组,未足月PROM组血清IL-6、IACM-1浓度高于足月PROM组,差异均有高度统计学意义(均P < 0.01).②PROM中度感染组母血IL-6、IACM-1水平高于PROM未感染组及PROM轻度感染组,差异均有高度统计学意义(均P < 0.01);PROM未感染组与PROM轻度感染组母血IL-6、IACM-1水平差异无统计学意义(P > 0.05).结论 感染与PROM密切相关,IL-6、IACM-1是诊断亚临床感染PROM的较好指标.