Objective To investigate the effects of all trans retinoic acid (ATRA) in anti peritoneal fibrosis of peritoneal dialysis. Methods Forty healthy male SD rats were selected, after numbered they were randomly divided into control group (intraperitoneal injection 10 mL/d normal saline+phosphate buffer diluting dimethyl sulfoxide with same volume as medicines), model group (intraperitoneal injection 4.25% peritoneal dialysis fluid 10 mL/d + phosphate buffer dilut-ing dimethyl sulfoxide with same volume as medicines), experiment group 1 (intraperitoneal injection 4.25%peritoneal dial-ysis fluid+ATRA 2 mg/kg), experiment group 2 (intraperitoneal injection 4.25%peritoneal dialysis fluid 10 mL/d+A-TRA 5 mg/kg), with 10 rats in each group; peritoneal membrane function of rats in four groups after 4 week were de-tected, changes of peritoneal fibrosis related cytokines in rats was detected by immunohistochemical method. Results In the fourth week, compared with the control group, peritoneal function indexes of UF, D/Purea, D1/D0 in model group and experiment group 1 declined (P< 0.05), those in experiment group 2 had no statistically significant difference (P>0.05). Compared with the control group, TGF-β1, CTGF,α-SMA expression of visceral peritoneum tissues in model group, experiment group 1 and experiment group 2 occured significantly changes (P<0.05), these in experiment group 1 and ex-periment group 2 were lower than those in model group (P<0.05). Compared with the control group, the expression of HGF, BMP-7, IL-6, VEGF in model group, experiment group 1 and experiment group 2 increased (P<0.05), these in exper-iment group 1 and experiment group 2 were lower than those in model group (P< 0.05). Conclusion ATRA can anti peritoneal fibrosis by adjusting the expression TGF-β1, CTGF,α-SMA and other cytokines.%目的：探讨全反式维甲酸(ATRA)在腹膜透析中的抗腹膜纤维化作用。方法选择40只雄性SD大鼠，编号后随机分为对照组(腹腔注射生理盐水10 mL/d+与药物等体积的磷酸盐缓冲液稀释的二甲亚砜)、模型组(腹腔注射4.25%的腹透液10 mL/d+与药物等体积的磷酸盐缓冲液稀释的二甲亚砜)、实验1组(腹腔注射4.25%的腹透液10 mL/d+ATRA 2 mg/kg)、实验2组(腹腔注射4.25%的腹透液10 mL/d+ATRA 5 mg/kg)，每组各10只；四组大鼠正常喂养4周后检测腹膜功能，并采用免疫组化法检测大鼠腹膜纤维化相关细胞因子的变化情况。结果实验第4周，模型组和实验1组的腹膜功能指标超滤量、透析液尿素浓度/血浆尿素浓度、初始腹透液葡萄糖浓度/透出液葡萄糖浓度测定值较对照组均有不同程度的下降(P<0.05)，实验2组与对照组比较，差异无统计学意义(P>0.05)。与对照组比较，模型组、实验1组、实验2组的脏层腹膜组织中转化生长因子-β1(TGF-β1)、结缔组织生长因子(CTGF)、α平滑肌肌动蛋白(α-SMA)免疫组化检测结果均发生显著的变化(P<0.05)，实验1组、实验2组较模型组的表达水平更低(P<0.05)。模型组、实验1组、实验2组肝细胞生长因子(HGF)、骨形态发生蛋白-7(BMP-7)、白细胞介素-6(IL-6)、血管内皮生长因子(VEGF)表达水平较对照组均显著升高(P<0.05)；实验1组、实验2组的HGF、BMP-7、IL-6、VEGF表达水平显著低于模型组(P<0.05)。结论 ATRA在腹膜透析中可以通过调节TGF-β1、CTGF、α-SMA等细胞因子的表达达到抗腹膜纤维化的目的。
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