目的:研制"一种医学检验微生物培养箱",并测试其在微生物检验中的应用效果.方法:选取在医院临床采集的160份微生物标本纳入研究样本组,采用研制的"一种医学检验微生物培养箱"进行微生物培育;同期另选取采集的160例微生物标本纳入对照样本组,采用不透光培养箱进行微生物培育,比较两组微生物检测阳性率、菌落总数及培养时间.结果:研究样本组血液、痰液、呼吸道和尿液标本微生物阳性检出率分别为40.00%、38.71%、58.82%和68.42%,均高于对照样本组的13.79%、19.40%、35.29%和22.22%,其差异有统计学意义(x2=10.5247,x2=5.8679,x2=4.0139,x2=7.9435;P<0.05);研究样本组血液、痰液、呼吸道和尿液标本菌落总数分别为(1.69±0.16)×109/cfu·ml-1、(1.59±0.23)×109/cfu·ml-1、(1.77±0.15)×109/cfu·ml-1和(1.73±0.14)×109/cfu·ml-1,均多于对照样本组的(1.51±0.26)×109/cfu·ml-1、(1.36±0.14)×109/cfu·ml-1、(1.53±0.27)×109/cfu·ml-1和(1.59±0.11)×109/cfu·ml-1,其差异有统计学意义(t=7.4580,t=10.8049,t=9.8287,t=9.9462;P<0.01);研究样本组细菌培养时间为(2.14±1.20)d,短于对照样本组的(3.06±1.14)d,其差异有统计学意义(t=7.0308,P<0.01).结论:在微生物检验中使用"一种医学检验微生物培养箱",可显著提升微生物阳性检出准确性,增加菌落培育量,且缩短培育时间,具有较高的临床应用价值.%Objective: To develop a microbiological incubator used in medical examination and explore its application and effect in bacteriological examination. Methods: 160 microbiological specimens which gathered from clinical examination of hospital were enrolled in research sample group that used the developed microbiological incubator to implement microbial cultivation, while other 160 specimens were enrolled in control group that used an opaque incubator to implement microbial cultivation at the same term. The positive rate of microbiological test, total bacterial count and incubation time of the two groups were compared and analyzed. Results: The positive rates of micrograms in blood, sputum, respiratory tract and urine of the research sample group were 40.00%, 38.71%, 58.82% and 68.42%, respectively, and all of them were significantly higher than that of control group, which positive rates for each item were 13.79%, 19.40%, 35.29% and 22.22%, respectively (x2=10.5247, x2=5.8679, x2=4.0139, x2=7.9435, P<0.05). The total bacterial counts of blood, sputum, respiratory tract and urine of the research sample group were (1.69±0.16)×109/CFU·ml-1, (1.59±0.23)×109/CFU·ml-1, (1.77±0.15)×109/CFU·ml-1and (1.73±0.14)×109/CFU·ml-1, respectively, and all of them were significantly more than that of control group, which total bacterial counts for each item were (1.51±0.26)×109/CFU·ml-1, (1.36±0.14)×109/CFU·ml-1, (1.53±0.27)×109/CFU·ml-1and (1.59±0.11)×109/CFU·ml-1, respectively (t=7.4580, t=10.8049, t=9.8287, t=9.9462, P<0.01). The microbial cultivation time of research sample group that was (2.14±1.20)d was significantly shorter than that of control group that was (3.06±1.14)d (t=7.0308, P<0.01). Conclusion: "The microbiological incubator for medical examination" is used in bacteriological examination can significantly enhance the accuracy of positive detection for microorganism, and significantly increase the number of cultivated bacterial colonies and shorten the cultivation time. Therefore, it has higher value in clinical application and should be popularized.
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