膀胱癌主要发生在膀胱粘膜上,是泌尿系统最常见的恶性肿瘤,其发病率和致死率较高.目前临床的技术手段对于膀胱癌的诊断和检测有诸多缺点和不足,例如检测过程有创伤,且敏感性和特异性均不高.本文设计并制备出一种新型的用于膀胱癌检测的微流控芯片,选取聚二甲硅氧烷作为芯片基片材料,采用模塑法制作芯片;使用硅烷化修饰在玻片上固定特异性抗体,并利用圆柱形阵列结构减缓流速以抓捕靶细胞.采用此芯片,从膀胱癌患者尿液样品中抓捕肿瘤细胞并经特异性荧光抗体进行鉴定.本文成功制备了无创膀胱癌检测的微流控芯片,为膀胱癌的检测和诊断提供了新的思路和有力的平台.%Bladder cancer mainly occurred on the bladder mucosa, and is the most common malignancy of the urinary system known as its high morbidity and mortality. However, many weaknesses and shortcomings still exist in current clinical diagnosis for bladder cancer, such as invasive detection, low specificity and sensibility. We proposed and designed a new type of microfluidic chip used for the detection of bladder cancer. Polydimethylsiloxane was used as the material of the microfluidic chip for production utilizing cast molding method. Specific antibody was fixed onto a glass slide after silanization modification and used to capture target cells with cylindrical micropattern which could slow down the cell velocity. Adopting the microfluidic chip, bladder cancer cells were captured from the urine sample of patients and identified by specific fluorescent antibody. The results showed that the microfluidic chip could specifically capture bladder cancer cells from urine of patients. This study has successfully established the microfluidic chip which detects bladder cancer noninvasively and provides new ideas and powerful platform for detection and diagnosis of bladder cancer.
展开▼
