刺老苞根皮水提物对骨折愈合相关因子受体表达的影响

摘要

目的:研究剌老苞根皮水提物在骨愈合修复过程中对相关因子受体表达的干预作用.方法:采用SD大鼠胫骨打孔的方法建立单因素骨折模型,分别于实验造模第14,28天取材,用免疫组织化学方法检测刺老苞根皮水提物在骨愈合修复过程中对成纤维细胞生长因子受体2(fibroblast growth factor receptor 2,FGFR2 )、血管内皮生长因子受体1、受体2(fms-like tyrosine kinase,Flt-1;fetal licer kinase,Flk-1)表达的影响.结果:14d时,利老苞根皮高剂量组增强了Flt-1、Flk-1的表达,以Flk-1更为显著,均高于模型组及刺老苞根皮低剂量组;刺老苞根皮高剂量组FGFR2有较高表达,与阳性对照药伤科接骨片组相近,高于模型组.28 d时,各用药组FGFR2、Flk-1、Flt-1表达减弱,模型组表达均高于各用药组.结论:刺老苞根皮水提物在骨愈合过程中对血管形成有明显促进作用,对成纤维细胞、成骨细胞、软骨细胞的增值和活性有较大影响,加速软骨修复,提前进入骨化和塑形期,促进骨折愈合.%Objective :To investigate the influence of aqueous extract of Aralia echinocaulis Hand.-Mazz on the expression of fracture healing-ralated factor receptors. Methods: Single factor model was set up in SD rat. Selecting 14 and 28 days in the experiment. Immunohistochemistry was employed to determine the expression of Fibroblast growth factor receptor 2 (FGFR2), Fms-like tyrosine kinase (Flt-1) and Fetal licer kinase (Flk-1) at 14 and 28 days after model establishing. Results:The expression of Flt-1 and Flk-1 at 14 days (the latter was more remarkable) were obviously promoted in High dose group of aqueous extract of Aralia echinocaulis Hand. -Mazz, and higher than that in normal group and model group. The expression of FGFR2 in the high dose group of Aralia echinocaulis Hand -Mazz was also promoted visibility,close to that in the compare group (traditional Chinese medicine) ,but higher than than in the model group. There was no significant difference among them. At 28 days,the expression of FGFR2,Flt-l and Flk-1 in all groups decreased except normal group,and got higher expression in model groups than each control groups. Conclusion: Aqueous extract of Aralia echinocaulis Hand.-Mazz can promote angio-genesis in fracture healing,improve the activity and aggregation of fibroblasts.osteoblasts and chondrocytes. It also helps to quicken ossification in the cartilage and promote fracture healing.