表皮生长因子受体及p53抑癌基因蛋白在老年妇女外阴营养不良及外阴癌中的表达及意义

摘要

目的探讨老年妇女外阴外阴营养不良组织中表皮生长因子受体(EGFR)及p53表达及临床意义.方法应用免疫组化法对老年妇女外阴正常外阴皮肤、慢性外阴营养不良、不典型增生和外阴癌组织进行标记.结果EGFR在正常外阴皮肤基底层有弱阳性表达,p53均阴性.EGFR在增生型、硬化苔癣型和混合型营养不良三者之间阳性率比较,差异均无显著性意义(P＞0.05);不典型增生和鳞癌的阳性率均明显高于各型营养不良(P＜0.05);而鳞癌与不典型增生之间差异无显著性意义(P＞0.05).p53基因蛋白在外阴不典型增生和外阴癌的表达率显著高于外阴营养不良病变的各型营养不良组织(P＜0.01).结论老年妇女外阴癌组织中p53与EGFR阳性表达率较高,EGFR与p53突变在老年妇女外阴癌的发生过程中起重要作用.%Objectiye: To investigate expression and significance of epidermal growth factor receptor(EGFR)and anti - oncogene protein p53 in vulva dystrophy tissues of old women. Methods: In old women issues of normal vulva skin, chronic vulva dystrophy, atypical hyperplasia and vulva carcinoma were marked by immunohistochemical technique. Results:Inferior positive expression of EGFR was found in normal vulva stratum basale, and p53 were all negative. There was no significant difference of positive rate of EGFR among hyperplasia, lichen sclerosis and mixed type dystrophy(P ＞ 0.05 ); the positive rates in atypical hyperplasia and squamous cell carcinoma were both significantly higher than the one of dystrophy in any types ( P ＜ 0.05 ); there was no significant difference of positive rate between hyperplasia and squamous cell carcinoma(P ＞0.05 ). The expression rate of p53 in vulva atypical hyperplasia and carcinoma was significantly higher than the one in dystrophy in any types(P ＜0.01 ). Conclusions:The expression rate of p53 and EGFR in vulva carcinoma tissues of old women is higher. EGFR and mutation of p53 do great help to the occurrence process of vulva carcinoma in old women.