Caspase 8和Caspase 3在TRAIL诱导神经母细胞瘤细胞凋亡中的作用

摘要

[Objective]To study the role of Caspase 8 and Caspase 3 in TRAIL-induced apoptosis of neuroblas-toma cell line CHP212 cells.[Methods]The effects of TRAIL and Caspase 8/Caspase 3 inhibitor+ TRAIL on the apoptosis of CHP212 cells were detected by flow cytometry.The relative Caspase 8/Caspase 3 activity was measured by colorimetric assay.The Morphology of the apoptosis cells were detected by using the transmission electron micro-scope (TEM).[Results]TRAIL could induce apoptosis of CHP212 cells in a dose dependent way.Caspase 8/Cas-pase 3 inhibitor could diminish the apoptosis of CHP212 ceils induced by TRAIL The relative Caspase 8/Caspase 3 activity of CHP212 cells increased gradually with the prolongation of TRAIL action time and reached the peak at 16h and 8 h respectively.Typical features of apoptosis were seen by TEM.[Conclusion]TRAIL could induce apopto-sis of CHP212 ceils through Caspase - transduced signal pathway with the increase of Caspase 8 and Caspase 3 ac-tivity.%目的 探讨Caspase 8和Caspase 3在肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导CHP212神经母细胞瘤细胞凋亡中的作用.方法 应用流式细胞仪检测TRAIL、Caspase 8/Caspase 3抑制剂+TRAIL对CHP212细胞的诱导凋亡作用.应用比色法测定Caspase 8、Caspase 3的相对活性.应用透射电镜对凋亡细胞进行形态学的观察.结果 TRAIL可诱导CHP212细胞的凋亡,并存在剂量依赖性;Caspase 8/Caspase 3抑制剂能抑制TRAIL对CHP212细胞的诱导凋亡作用.随TRAIL作用时间的延长,Caspase 8、Caspase 3的活性逐步升高,分别于作用16h、8h后达高峰.透射电镜可见到典型的细胞凋亡特征.结论 TRAIL通过Caspase信号传导通路诱导CHP212细胞凋亡并伴随Caspase 8和Caspase 3活性的增高.