Nutlin-3对紫外线诱发的人黑素细胞DNA氧化损伤的保护作用

摘要

Objective: To evaluate the protective effects of Nutlin - 3 on oxidative DNA damage of the melanocytes after ultraviolet exposure. Methods: Normal functioning MC1R melanocytes were pretreated with a - MSH, followed with ultraviolet irradiation ( UVB, 105 mJ/cm2), Nutlin - 3 (10μM) and UVB plus Nutlin - 3, respectively. Tailing rates, tail movements and tail torque of each group were analyzed with Comet Assay. Results: Tailing rates, tail movements and tail torque were the highest in the UVB group ( P < 0.05) , and were significantly lower in the UVB + Nutlin - 3 group than in the UVR group ( P < 0.05) . Conclusion: Nutlin - 3 can reduce melanocytic DNA damage induced by UVB irradiation through a non - melanogenesis route.%目的:评价Nutlin -3对紫外线诱发的人黑素细胞DNA氧化损伤的保护作用.方法: a-MSH预处理MC1R功能正常的人黑素细胞,分组予以紫外线照射(UVB,105 mJ/cm2)、Nutlin -3(10μM)和UVB+ Nutlin -3不同干预方法,并采用彗星实验确定各组DNA托尾率、尾距和尾矩数值.结果:UVB组DNA托尾率、尾距和尾矩数值高于其他各组(P＜0.05),UVR+ Nutlin -3组明显低于UVB组(P＜0.05).结论:Nutlin -3能够减少UVB诱导黑素细胞DNA损伤.