细胞外基质蛋白1对HaCaT细胞增殖及细胞周期的影响

摘要

Objective: To determine the effect of extracellular matrix proteins- 1 (ECM1) on the proliferation and cell cycle of HaCaT cell. Methods: the supernatant containing ECM1 of pEGFP - N2 - ECM1 transfected MCF - 7 cell was added to HaCaT cells. MTT method was used to detect the proliferation of HaCaT cells and FCM method was used to measure the change of cellular cycle. Results: ECM1 showed positive effect on the proliferation after 48hs, when treated with different concentration. Compared with control group, the cells in G, phase decreased, while cells in C2 and S phases increased. Conclusion: ECM1 can enhance the proliferation of HaCaT cell.%目的:确定细胞外基质蛋白1(ECM1)对HaCaT细胞增殖及细胞周期的影响.方法:将转染pEGFP- N2 - ECM1的含有ECM1的MCF-7细胞上清液作用于HaCaT细胞.MIT法检测HaCaT细胞的增殖,FCM法测定细胞周期变化.结果:与对照组相比,经ECM1作用48h后,细胞G1期比例显著降低,S期与G2期比例则显著升高.结论:ECM1对培养的HaCaT细胞具有诱导增殖的作用.