填精补髓、活血化痰开窍法对血管性痴呆大鼠海马组织BDNF和bFGF含量的影响

摘要

目的：探讨填精补髓、活血化痰开窍法对血管性痴呆（VD）大鼠海马组织脑源性神经营养因子（BDNF）、碱性成纤维细胞生长因子（bFGF）含量的影响。方法采用双血管阻塞（2VO）改良法复制VD大鼠模型，以盐酸多奈哌齐片为阳性对照组，观察填精补髓、活血化痰开窍法对大鼠海马组织BDNF及bFGF含量的影响。结果与假手术组比较，模型组大鼠海马组织BDNF和bFGF含量升高（P＜0.05）；与模型组比较，阳性对照组，中药高剂量组，中药中剂量组海马组织BDNF含量升高（P＜0.05），阳性对照组，中药高剂量组海马组织bFGF含量升高（P＜0.05）。结论填精补髓，活血化痰开窍法可通过提高VD大鼠海马组织BDNF和bFGF含量，改善其学习与记忆能力。%Objective To explore the effect of tianjingbusui huoxuehuatankaiqiao on the contents of basic fibroblast growth factor (bFGF) and brain-derived neurotrophic factor (BDNF) in hippocampus of vascular dementia(VD) rats.MethodsThe vessel occlusion (2VO) was modified to establish the VD model,and the contents of bFGF and BDNF in hippocampus were observed.Results Compared with normal group,the contents of bFGF and BDNF were increased(P<0.05) in model group. Compared with model group,the contents of BDNF were increased(P<0.05) in the high, middle dose of traditional Chinese medicine groups and the donepezil group, the contents of bFGF were increased(P<0.05)in the high dose of traditional Chinese medicine groups and the donepezil group.ConclusionThe tianjingbusui and huoxuehuatankaiqiao could increase the contents of bFGF and BDNF,and improve the learning and memory capability.