HILIC测定金丝小枣中核苷酸含量

摘要

In this paper, we used cv. Jinsixiaozao as experimental material to determine its nucleotide conten. We have established a method, that is ultrasonically assisted extraction combined HILIC. Then we optimized the method. The results proved that the method had good precision, reproducibility and stability.%本文以金丝小枣为试材，采用超声波辅助提取法对样品进行优化提取，然后进行亲水色谱分析，测定样品中9种核苷酸的含量。实验中采用HILIC XBridgeTMAmide色谱柱，以乙腈-0.3%乙酸水溶液为流动相，梯度洗脱，流速为0.8mL/min，柱温为室温，检测波长260nm，进样量30滋L；对金丝小枣中9种核苷酸含量进行分析。结果表明，该分析方法具有良好的精密度、重现性和稳定性，适合于金丝小枣中核苷酸含量的测定，为枣的质量控制研究提供了方法和技术参考。