Two anaerobic sequenced batch reactors (ASBR) fed with glucose and sodium acetate were applied to investigate the effect of biogas recirculation, i.e. recycling the biogas with and without hydrogen removal, on their performance. Acetate metabolic rate, effluent properties and microbial morphologies were studied. Meanwhile, molecular weight (MW) distribution and fluorescence feature of soluble microbial products (SMP) were analyzed by size exclusion chromatography and fluorescence excitation-emission matrix spectroscopy. Gas regulation improved the mixing condition,which accelerated the anaerobic reaction rates such as methanogenesis. The residual volatile fatty acids (VFAs) declined from 238.2mg/L (without hydrogen removal) and 129.6mg/L (with hydrogen removal) to 8.5mg/L and 8.2mg/L,respectively. Biogas recirculation eventually decreased the SMP content, which fell to 36.8％ and 59.2％ of the initial values respectively after 45days. Hydrogen removal promoted acetogenesis and acetate oxidation, and obviously changed the concentrations of methanogenic precursors and the microbial morphologies, It also promoted the conversion of high MW-SMP to low MW-SMP. Gas recirculation improved the quality of the effluent, while hydrogen removal accelerated the metabolism of acetate, thus is expected to facilitate the release of methanogansis from acidification and enhance the anaerobic conversion of organics.%采用厌氧序批式反应器,研究了气体循环(包括气体直接循环和脱除气体中的氢气后循环)对以葡萄糖和乙酸钠配制的模拟废水在高温厌氧消化过程中乙酸代谢速率、出水性质及微生物相的影响,并采用尺寸排除色谱和三维荧光光谱技术,对溶解性微生物产物(SMP)的分子量分布和荧光物质组成进行了分析.结果显示,气体循环使得出水中残余挥发性脂肪酸的浓度由238.2mg/L(未脱氢)和129.6mg/L(脱氢)分别减少至8.5mg/L和8.2mg/L,并最终降低了SMP产量,45d时分别降至气体循环前的36.8%和59.2%.脱氢气体循环促进了乙酸化和乙酸氧化,导致了微生态环境和甲烷化基质浓度的差异,促进了高分子量SMP向低分子量SMP的转化,污泥中的微生物形态亦发生了较大变化.可见气体循环改善了基质与微生物的混合状况,改善了出水水质,而脱氢气体循环加速了乙酸的代谢,有望更快速地解除易降解有机物高温厌氧消化过程中的酸抑制,提高厌氧消化效率.
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