抗牛支原体及多杀性巴氏杆菌二联卵黄抗体的制备及初步临床应用观察

摘要

本研究将自制牛支原体和牛源荚膜血清A型多杀性巴氏杆菌灭活菌苗分别免疫产蛋鸡，并检测效价，采用卡拉胶结合硫酸铵沉淀法提取IgY，观察抗牛支原体及多杀性巴氏杆菌二联高免卵黄抗体制剂在犊牛中应用的效果，并进行临床应用研究。结果显示，牛支原体在首免后第60d抗体效价达到高峰，经ELISA检测效价为1：128000；牛多杀性巴氏杆菌首免后第50d抗体效价达到高峰，经IHA检测效价为1：1024。临床初步应用表明，二联卵黄抗体对犊牛肺炎的预防和治疗均有一定作用。%To prepare of yoly against Mycoplasma bovis and Pasteurella multocida pneumoniae and observe the effect on clinical application.Through self-made inactivated M.bovis and bovine serum capsular type A Pasteurella multocida vaccine were immunized hens, and detect the titer, the method of carrageenan combined with ammonium sulfate to extract IgY, the antibody formulations applied to the calf and observe the effect. Finally to do clinical study. Results showed that M.bovis yolk antibody titer reached the highest at the 60th day after ifrst immunization and Yolk antibody titers 1:128000, which was detected by the method of indirect ELISA, Pasteurella multocida pneumoniae yolk antibody titer reached the highest at the 50th day after ifrst immunization and Yolk antibody titers 1:1024 ,was detected by the method of IHA. Preliminary clinical application showed that the duplex yolk antibodies have effect to calf pneumonia on prevention and treatment .