为调查副结核病在西藏地区牦牛群感染及流行情况，采用酶联免疫吸附试验（Enzymelinkedimmunosorbentassay,ELISA），对2012~2014年采自西藏部分地区的468份牦牛血清进行副结核分枝杆菌抗体检测。结果检出9份阳性血清，2份可疑血清，总体阳性率为1.92%（9/468）；2012~2014年阳性率分别为1.27%(2/157)、2.42%(4/165)和2.05%(3/146)。结果表明，西藏地区牦牛存在副结核分枝杆菌的感染，在牛群中呈较低的流行率。该调查结果可为当地的牦牛产业疾病防控提供依据。%The seroprevalence of paratuberculosis Mycobacterium avium subspecies Paratuberculosis (Map) infection in yaks was conducted in Tibet. A total of 468 serum samples were collected from 2012 to 2014, and tested for antibodies by a commercial enzyme-linked immunosorbent assay (ELISA, IDEXX). The results showed that 1.92%(9/468) were calculated as positive and 2 samples were suspected for Map.Apparent prevalence (AP) of Map in Tibet was 1.27%, 2.42%and 2.05%in 2012, 2013 and 2014, respectively. Our study conifrmed that the yaks in Tibet had been affected by Map. The objective of this research was to investigate the seroprevalence of Map infection in yaks in Tibet, and to provide the theoretical basis for disease prevention.
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