紫外-光复活诱变选育发酵玉米蛋白粉饲料的产朊假丝酵母

摘要

为了更好地利用微生物发酵玉米蛋白粉生产新型蛋白饲料,该研究以产朊假丝酵母(Candida utilis)作为出发菌株,对其进行紫外-光复活诱变,以其蛋白酶活力为评价指标,筛选酵母菌株,并进行遗传稳定试验.结果表明,经紫外-光复活诱变试验筛选出一株突变株FC-20.14,中性蛋白酶活力为64.18 U/mL,较出发菌株酶活力提高1.93倍,经遗传稳定试验结果表明其遗传稳定,可将其用于发酵玉米蛋白粉饲料.%In order to make better use of microbial fermented corn protein powder to produce new protein feeds, using Candida utilis as starting strain,the strain was treated by UV photoreactivation mutagenesis. Taking the protease activity as an evaluation index, the yeast strains were screened, and the genetic stability tests were carried out. The mutant strain FC-20.14 was screened through the UV photoreactivation mutagenesis tests. Its neutral protease activity was 64.18 U/ml, which was 1.93 times higher than that of the starting strain. The results of genetic stability tests indicated that the genetics were stable. It can be used for the production of fermented corn protein powder feed.