Pyruvate is the important intermediate by organic synthesis, which has widespread application in medicine, cosmetics, chemical production. Microbial production is one of the main methods to obtain pyruvate. To improve the viability and fermentation performance of the strain, shorten the fermentation time, and decrease energy consumption of industrial production, here, adaptive evolution of Torulopsis glabrata under the condition of three-step increased growth temperature was applied to improve the strain performance. A new T. Glabrata TIB-G90 mutant was obtained which accumulates the same amount of pyruvate with less fermentation time at 40℃(24h) than 30℃(36h), was screened out. This study showed the potential of microbial adaptive evolution to improve microbial production of metabolites.%丙酮酸是重要的有机合成中间体,广泛应用于医药、化妆品、化学生产等工业中.发酵法生产丙酮酸是获得丙酮酸的主要方法之一,为了提高光滑拟球酵母菌的生长和发酵能力,缩短发酵周期,减少生产过程中能量消耗,改善丙酮酸生产性能,选取了一株产丙酮酸的光滑球拟酵母菌3672 CGMCC No0879进行高温适应进化,得到一株相对于出发菌株,能够在40℃生长良好,且葡萄糖代谢率快,丙酮酸产量稳定的耐高温菌株光滑球拟酵母TIB-G90 CGMCC No5434,在40℃条件下发酵24h时,丙酮酸积累量可达到28g/L左右,而出发菌株在30℃条件下需要发酵36h才能达到相同的丙酮酸积累量.
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