克雷伯氏菌发酵条件的响应面设计

摘要

To enhance 1,3-propanediol (1,3-PD) production from glycerol, fermentation conditions were optimized by response surface methodology (RSM) based on central composite design (CCD). A statistical model for the individual and interactive effects of glycerol, (NH4)2SO4, pH value, fermentation time and temperature on the production of 1,3-PD was developed. Optimal conditions were as follows: glycerol 49.9g/L, (NH4)2SO4 5.28g/L, pH value 7.19, fermentation time 84h and temperature 36.lt. Under these optimal conditions, the predicted production of 1,3-PD by statistical model was 19.03g/L. The experimental production of 1,3-PD yielded 18.33g/L, which was closed to the predicted value of the statistical model.%以中心组合设计为基础利用响应面法,对克雷伯氏菌利用甘油生产l,3-丙二醇(1,3-PD)的培养条件进行优化,建立了以甘油、硫酸铵、pH值、发酵时间、和发酵温度对l,3-PD发酵生产的单个因素和交互影响的数学模型,得到发酵生产l,3-PD的最佳条件为:甘油49.9g/L；硫酸铵5.28g/L；pH值为7.19;培养时间84h;温度36.1℃.在此条件下,模型预测1,3-PD最大产量为19.03g/L.并在此条件下进行实际实验,1,3-PD的产量为18.33g/L,与模型预测接近.