一株产纤维素酶曲霉菌白酒丢糟发酵培养基的优化

摘要

为充分利用白酒厂丢糟营养成分,以白酒厂大曲中分离筛选到的一株具有较高纤维素酶活性曲霉菌为菌种,用白酒丢糟为主要原料,对该曲霉菌固态发酵产纤维素酶的培养基进行了优化.结果表明,以白酒丢糟为主要碳源、麸皮为诱导氮源、(NH4)2SO4为速效氮源时,培养基组成以速效氮源(硫酸铵)与诱导氮源(麸皮)比例约为2∶3,碳源(丢糟)与诱导氮源(麸皮)比例约为2∶1,固液比为1∶5时,其纤维素酶活力最高,可达4.07U/mL.%To make fall use of the nutrients in distiller's grains (the wastes of Chinese liquor making), a high cellulase-producing Aspergillus sp. Was isolated from a 31 -day Daqu for the production of cellulase on distiller's grains by solid state fermentation. The fermentation medium was optimized taking distiller's grains as main carbon source, wheat bran as controlled-release nitrogen source and (NH4)2SO4 as fast-release nitrogen source. The results showed that the highest cellulase activity reached 4.07U/ml when the ratios of (NH4)2SO4 to wheat bran, distiller's grains to wheat bran, and solid to liquid were 2:3,2:1 and 1:5, respectively.