In this paper, the microelements of worker bees with three growth stages(emerge workers, nurse bees and forge bees) in Apis cerana cerana and Apis mellifera ligustica were studied by laser induced breakdown spectroscopy. The results showed that the physique of emerge workers, nurse bees and forge bees were rich in Fe, Ca,Ba, Li, K,Na, while the content of Ca was higher than that of other elements. In addition,the relative content of the elements in different stages of the same species or the same stage of different species was different. The experimental results also showed that laser induced breakdown spectroscopy could be used for the analysis of microelements in bees physique, and it was a rapidly qualitative and comparatively quantitative method.%试验以中华蜜蜂和意大利蜜蜂为材料,应用激光诱导击穿光谱技术对两蜂种工蜂3个生长阶段(初生蜂、哺育蜂、采集蜂)体质主要微量元素进行初步定性和半定量分析.结果表明,中华蜜蜂和意大利蜜蜂的初生蜂、哺育蜂及采集蜂体质中富含Fe、Ca、Ba、Li、K、Na等元素.其中Ca元素的相对含量最高,明显高于其他几种元素的相对含量.另外,同种蜜蜂不同生长阶段及不同蜂种同—生长阶段中各元素的相对含量也不一样.因此,激光诱导击穿光谱技术能够用于检测蜜蜂体质中的微量元素,并具有快速的定性和半定量分析能力.
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