巴美肉羊发情期外周血E2和P4浓度变化规律与排卵数关系研究

摘要

绵羊在发情周期中各生殖激素浓度变化与卵泡发育、成熟和排卵有着密切的关系.为了研究巴美肉羊血清生殖激素的动态变化及其与排卵数关系,试验采用电化学法,测定了12只成年母羊发情期血清中2种类固醇激素(E2和P4)的浓度水平,分析其动态变化规律,并用SAS 9.0的方差分析程序分析激素浓度与排卵数的关系.结果表明,两种激素在排单卵组和排双卵组绵羊间变化规律不同,E2在排单卵组表现为先下降后升高的变化趋势,在排双卵组表现为持续下降趋势；P4在排单卵组表现为持续上升的趋势,在排双卵组为先上升后下降的变化趋势.排单卵和排双卵组绵羊在各时间点的E2和P4激素浓度差异均不显著(P＞0.05).%Concentration changes in each reproductive hormones during sheep oestrus cycle are closely related with follicu-lar development, maturation and ovulation. In order to study the dynamic changes of the serum reproductive hormones of the Bamei Mutton sheep and their relationship with ovulation rate, the electrochemical method was used to measure serum concentrations of two steroid hormones (E2 and P4) for 12 adult ewes during estrus, and the variance analysis procedures of the SAS 9. 0 were used to analyze the relationship between hormone concentration and ovuiation rate. The results showed that variation of the two hormones were different between the laying-single-egg group and the laying-double-eggs group sheep. Concentration of E2 firstly decreased and then increased in laying-single-egg group, and continuously declined in laying-double-eggs group. Concentration of P4 continuously increased in laying-single-egg group, and firstly increased and then decreased in laying-double-eggs group. Concentration differences of E2 and P4 at each time point between the two groups were not significant(P>0. 05) .