影响猪卵母细胞胞质内单精子注射效果的因素

摘要

The objective of this study was taken to investigate the effects of different additive of maturation media and sperm pretreatment and activation methods on ICSI embryos. The results showed that the development of ICSI embryos got improving obviously by adding 30 ng/mL EGF in maturation medium(P<0. 05) ,it had no significant effect for ICSI embryos between ultrasonic treatment and microinjection in sperm(P>0. 05) , and the electro-activation was suitable for ICSI embryos.%本试验探讨了成熟培养液中不同添加物、精子的处理方法及胞质内单精子注射(intracytoplasmic sperm injection,ICSI)胚胎激活方法3个方面,研究了影响猪卵母细胞胞质内单精子注射效果的因素.结果表明,在体外成熟液中添加30 ng/mL上表皮生长因子(epidermal growth factor,EGF)可以明显促进ICSI胚胎的后续发育(P＜0.05)；精子的超声波处理和显微操作处理两种方法对ICSI受精卵发育的影响不明显(P＞0.05)；电激活比较适合于ICSI胚胎的激活.