表观健康猪群携带猪链球菌2型毒力因子的分布特征

摘要

To investigate the distribution of virulence factors of Streptococcus suis Serotype 2(SS2),four virulence factors were detected by multiplex PCR amplification,namely capsular polysaccharide(Cps),muramidase-released protein(Mrp),extracellular protein factor(EF),suilysin(Sly). The results confirmed that in 11 strains from slaughtered healthy pig’s tonsil,there are 9 strains (81.8%)belong to Mrp+epf +Sly+and Mrp+epf +Sly-,and 2 strains(18.2%)belong to Mrp-epf-Sly-. The results showed that the prevalent strains of SS2 in Qinzhou were highly virulent strains which contain all four virulence factors.%　　为了研究钦州市猪链球菌2型(SS2)的毒力因子分布特征，采用多重PCR方法对从我市健康猪扁桃体分离的11株SS2相关毒力基因——荚膜多糖(Cps2J)，溶菌酶释放蛋白(Mrp)，胞外蛋白因子(EF)，溶血素(Sly)，进行毒力基因检测.检测结果显示：毒力基因型为Mrp+epf+Sly+共有8株，毒力基因型为Mrp+epf+Sly-共有1株，这两种毒力基因型可归为强毒力基因型，占此次健康猪源毒株的81.8%；另外Mrp-epf-Sly-共有2株，属于弱毒和无毒基因型，占健康猪源毒株的18.2%.通过结果分析，预示我市目前SS2的主要流行菌株是同时具有4种毒力因子的高致病性菌株.