海洋来源真菌Hypocrea virens菌丝体的化学成分研究

摘要

Eight compounds were isolated from the mycelium of marine-derived fungus Hypocrea virens by using silica gel,Sephadex LH-20,open ODS column chromatography and HPLC. On the basis of spectral data and physicochemical properties,their structures were identified as l(10→6)abeo-(22E,24R)-ergosta-5,7,9,22-tetraene-3α, 1 lα-diol(I), 24-methylenelanostane-8-ene-3β-ol(II), (22E,24R)-ergosta-5,7,22-triene-3β-ol (III), (22E,24R)-ergosta-8,22-diene-3β,5α,6β,7α-tetraol(IV), 5a, 9a-epidioxy-(22E,24R)-ergosta-7, 22-diene-3β,6β-diol(V),5α,8α-epidioxy-(22E,24R)-ergosta-6,22-diene-3β-ol( VI), (22E,24R)-ergosta-7,22-diene-3β,5α, 6β-tri-ol(VIII),3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-diene-6-one(VIII).Compounds I , II ,IV,V,VIII were isolated from the Hypocrea genus of marine-derived fungus for the first time,and compounds III,VI,VIII were isolated from the marine-derived fungus Hypocrea virens for the first time.%采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、开放ODS柱色谱以及HPLC等对海洋来源真菌Hypocrea virens的菌丝体进行化学成分分离,通过理化性质和波谱数据鉴定化合物的结构.从菌丝体的丙酮提取物中分离得到8个化合物,分别鉴定为1(10→6)Abeo-(22E,24R)-ergosta-5,7,9,22-tetraene-3a,11α-diol(Ⅰ)、24-甲烯基羊毛脂烷-8-烯-3β-醇(Ⅱ)、(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(Ⅲ)、(22E,24R)-麦角甾-8,22-二烯-3β,5α,6β,7α-四醇(Ⅳ)、5α,9a-过氧-(22E,24R)-麦角甾-7,22-二烯-3β,6β-二醇(Ⅴ)、5α,8α-过氧-(22E,24R)-麦角甾-6,22-二烯-3β-醇(Ⅵ)、(22E,24R)-麦角甾-7,22-二烯-3β,5a,6β-三醇(Ⅶ)、3β,5α,9α-三羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(Ⅶ),其中化合物Ⅰ、Ⅱ、Ⅳ、Ⅴ、Ⅷ均为首次从该属真菌中分离得到,化合物Ⅲ、Ⅵ、Ⅶ为首次从该种真菌中分离得到.