含锰功能基包覆二氧化硅颗粒的SOD活性研究

摘要

以5-甲基水杨醛和乙二胺为原料合成席夫碱化合物,用NaBH4将其还原,还原产物与3-溴丙基三甲氧基硅烷发生亲核取代反应,经水解反应后和锰离子配位得到一种锰配合物修饰的功能基固体颗粒。采用红外光谱、热重分析及X-射线能谱对其结构进行表征,用改进的四氮唑蓝法对其 SOD活性进行测定。结果表明,锰配合物修饰的功能基固体颗粒的 SOD活性单位 IC50为0.055 g·L-1,其具有 SOD活性和循环使用性。%In this paper,functionalized solid particles modified by Mn (Ⅱ)complexes were synthesized through the following steps:firstly,a Schiff base compound was synthesized using 5-methyl salicylaldehyde and ethylenediamine as raw materials,then it was reduced by NaBH4 ,and a nucleophilic substitution reaction was carried out between the reduced product and 3-bromopropyl trimethoxysilane,followed by coordination reaction with Mn(Ⅱ)ions after hydrolyzation.The product was characterized by infrared spectroscopy(IR),thermo-gravimetric analysis(TGA)and X-ray energy dispersive spectrometer(EDS).The SOD activity of the sample was measured by using a modified blue tetrazoliym method.Results showed that the SOD activity unit IC50 of the sample was 0.055 g·L-1 .The product exhibited SOD activity and reusability.