共生真菌巴西类壳小圆孢中环二肽类代谢产物研究

摘要

Nine cyclic dipeptides secondary metabolites were isolated from the symbiotic fungus paraconiothyrium brasiliense, by normal phase silica gel,Sephadex LH-20 chromatography and reverse phase HPLC. Their structures were elucidated as cyclo-( L-Leu-L-Tyr)(1),cyclo-(L-Trp-L-Pro)(2),cyclo-(L-Pro-L-Phe)(3),cyclo-(L-Pro-L-Tyr)(4),cyclo-(L-Pro-L-Leu)(5),cyclo-(L-Leu-trans-4-hydroxy-L-Pro)(6),cyclo-(L-Ile-L-Pro)(7),cyclo-(L-Val-L-Pro)(8),cyclo-(L-Leu-L-Ile)(9)by NMR,MS and physicochemical properties. All the compounds were isolated from the symbiotic fungus paraconiothyrium brasiliense for the first time.%利用正相和反相柱色谱,Sephadex LH-20凝胶柱色谱及反相HPLC等方法从昆虫共生真菌Paraconiothy-rium brasiliense的发酵产物中分离得到9个环二肽类化合物,经NMR,MS及理化性质等方法,结构确定为环(L-亮氨酸-L-酪氨酸)(1),环(L-色氨酸-L-脯氨酸)(2),环(L-脯氨酸-L-苯丙氨酸)(3),环(L-脯氨酸-L-酪氨酸)(4),环(L-脯氨酸-L-亮氨酸)(5),环(L-亮氨酸-反式-4-羟基-L-脯氨酸)(6),环(L-异亮氨酸-L-脯氨酸)(7),环(L-缬氨酸-L-脯氨酸)(8),环(L-亮氨酸-L-异亮氨酸)(9)。所有化合物均为首次从该属菌种中分离得到。