Based on anion and cation electrostatic load principle, using sodium alginate and chitosan as raw materials,sustained-release microspheres were obtained by the drip method and layer-by-layer self-assembly. The microspheres were then put into a certain percentage of sodium alginate-chitosan solution,and a new kind of scaffold with microspheres embedded in the spongy structure called sustained-release microsphere-type scaf-folds was obtained. The influences of the component ratio of the sustained-release microspheres on the porosi-ty,contraction, hydrophilicity and degradation properties of the sustained-release microsphere-type scaffolds were investigated. The pore size was between 140 and 200 μm analyzed by scanning electron microscopy (SEM). There was no difference in the cytotoxicity text of the scaffold's leachate between the experimental group and the control group.When the component ratio of microspheres was 10%,the scaffolds had the highest porosity ratio(68.2%—70.8%),the lowest contraction percentage(4.4%—5.2%)and the best hydrophilic-ity. The less microspheres the scaffolds had,the faster the degradation rate was.For preventing the degradation rate too fast and carrying more medicines,the scaffolds,whose component ratio of microspheres was 20%,are much better. The microsphere-type scaffold material has good plasticity before freeze-drying. The microsphere-type scaffolds provided an example of the combination with sustained-release systems and existing porous scaf-fold materials.%利用海藻酸钠和壳聚糖2种原料,采用阴阳离子静电复合原理,通过滴注法层层自组装成可搭载药物的缓释微球,再按一定比例与海藻酸钠-壳聚糖溶液混合制成缓释微球型支架材料,将缓释微球结构嵌入疏松多孔海绵状结构中.研究了缓释微球的组分比对缓释微球型支架材料的孔隙率、收缩率、亲水性及降解性能的影响;扫描电子显微镜照片显示,微球结构相对完整,多孔海绵状结构孔径为140~200 μm;支架浸出液细胞毒性检测实验组对照组未见差异.缓释微球体积所占比例即组分比为10%的缓释微球型支架材料孔隙率最高为68.2%~70.8%,亲水性最好,收缩率最低为4.4%~5.2%;支架降解速率随缓释微球组分比升高而减慢,组分比为20%的缓释微球型支架材料综合性能更优;缓释微球型支架材料冻干成型前为液态,具有良好可塑性.缓释微球型支架材料为缓释系统与多孔支架材料有机结合提供了新思路.
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