不同脱毒方法对玉米秸秆水解液酒精发酵的影响

摘要

利用湿热预处理(195℃,15 min)后的玉米秸秆水解液,考察了3种不同脱毒方法(中和法、饱和生石灰法和Na2SO3法)对水解液中的抑制剂的去除效果,研究了树干毕赤酵母(Pichia stipitis 58376)对脱毒后的水解液酒精发酵情况.结果表明:玉米秸秆水解液经过3种方法脱毒处理后,醛类抑制荆(糠醛和5-羟甲基糠醛)平均减少41%,总酚类最高去除28.4%,酒精得率都得到明显提高.最佳的脱毒方法是饱和生石灰法,理论酒精得率达到69.31%,对应的酒精浓度和生产效率分别为12.2 g/L和0.056 g/(L·h).饱和生石灰法是一种有效实用的脱毒方法.%The influence of three different detoxification methods (neutralization, overliming and Na2SO3 addition ) on inhibitors were evaluated by using corn stover hydrolysate prepared with hydrothermal pretreatment ( 195 ℃, 15 min ) . Ethanol fermentability of detoxified corn stover hydrolysate was investigated by Pichia stipitis 58376. The results showed that all the employed detoxification methods resulted in a 41％ reduction in average total furans and highest 28.4％ reduction in total phenols. Fermentation performance was greatly enhanced by employed detoxification methods.Ethanol yield of 69.31％ of the theoretical value based on reducing sugar was obtained by overliming.The corresponding ethanol concentration and volumetric productivity were 12.2 g/L and 0.056 g/( L·h ).Overliming was the most efficient detoxification method.