吖啶类荧光探针与蛋白质相互作用的研究

摘要

The paper aims to research on the mechanisms of binding and interaction between 3 new types of acridine fluorescence probes, namely, NCAF, NAFA&DNAF and bovine serum albumin(BSA). It compared the 3 types of acridine fluorescence probes one another in terms of self-aggregation, constant of binding with BSA (KA), number of binding sites (n), thermodynamic parameters △H,△G and△S, energy conversion efficiency (E) and binding distance R0, and analyzed all the experiment data. It researched on both of quenching system against the quenching of protein intrinsic fluorescence and type of primary action force for the said 3 types of acridine fluores-cence probes, and thus provides certain experimental and theoretical support for further researching on new bio-logical probes and their application to identifying and analyzing large biological molecules.%文章研究3种新型吖啶类荧光探针（N-（2-二甲氨基）乙基-9-氯吖啶-4-甲酰胺（NCAF）、9-[（N-2-二甲氨乙基）吖啶-4-甲酰胺]-α-丙氨酸（NAFA）和4,9-二[N-（2-二甲氨基）乙基]-9-吖啶胺-4-甲酰胺（DNAF））与牛血清白蛋白（BSA）的相互结合作用机理。分别对3种吖啶类探针自聚集情况、与牛血清白蛋白结合常数KA和结合位点数n、热力学参数H、G以及S、能量转移效率E和结合距离R0进行比较，并对实验数据进行分析。研究了3种吖啶类探针对蛋白质内源荧光猝灭的猝灭机制和主要作用力类型，为进一步研究新的生物探针及其在生物大分子识别分析应用提供了一定的实验和理论支持。