A method for the determination of matrine and oxymatrine in sophoraflavescens by reversed phase liquid chromatography was established. The extraction solvent was ethanol, C18 column was selected, methanol-water-three ethylamine solution (55:45:0.2) was used as the mobile phase with theflow rate of 0.8 mL/min,and the UV detector wavelength was 210 nm. The concentration of matrine and oxymatrine was linear with its chromatographic peak area in the range of 10-200 mg/L,the linear correlation coefficients were more than 0.999, and the detection limits were 0.05, 0.1 mg/L, respectively. The recovery rates of matrine and oxymatrine were 98.47%, 97.89%,and the relative standard deviations of determination results were 1.32% and 1.08%(n=6), respectively. The method has the advantages of simple operation, less interference and high sensibility.%建立反相液相色谱法测定苦参中苦参碱和氧化苦参碱含量的方法.提取溶剂为乙醇,选择C18色谱柱,以甲醇-水-三乙胺溶液(55:45:0.2)为流动相,流量为0.8 mL/min,紫外检测器波长为210 nm.苦参碱和氧化苦参碱的质量浓度在10~200 mg/L范围内均与其色谱峰面积呈良好的线性,线性相关系数均大于0.999,检出限分别为0.05,0.1 mg/L.苦参碱和氧化苦参碱的加标回收率分别为98.47%,97.89%,测定结果的相对标准偏差分别为1.32%,1.08%(n=6).该方法操作简便,干扰少,灵敏度高.
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