23株海洋真菌的分子鉴定及其抗植物病原真菌和细胞毒活性研究

摘要

从南海沉积物中分离得到23株海洋真菌，通过ITS测序进行鉴定。以新月弯孢霉（Curvularia lunata）、柱枝双胞霉（Cylindrocladium scoparium）、链格孢（Alternaria alternata）、胶孢炭疽菌（Colletotrichum gloeosporioides）为受试植物病原真菌，以神经胶质瘤细胞（SF-268）、乳腺癌细胞（MCF-7）、大细胞肺癌细胞（NCI-H460）和肝癌细胞（HepG-2）为受试肿瘤细胞，分别采用生长速率法和SRB法对这些菌株的发酵液粗提物进行抗植物病原真菌和细胞毒活性测试，发现11个菌株的粗提物在浓度为50 mg/mL时，对至少1种受试植物病原真菌的抑制率在50%以上，有9个菌株的粗提物在浓度为100μg/mL时，对至少1种肿瘤细胞株的抑制率在80%以上，其中菌株Eupenicillium sp. FS100、Penicillium sp. FS105、Dichotomomyces cejpii FS110、Acaromyces ingoldii FS121对植物病原真菌和（或）肿瘤细胞具有明显的抑制活性。%Twenty three marine fungal strains were isolated from the sediments of the South China Sea and identified by intergenic transcribed spacer(ITS)sequencing. The fermentation broth extracts of these marine fungal strains were tested for their inhibitory activities against growth of 4 plant pathogenic fungi, namely Colletotrichum gloeosporioides, Alternaria alternate, Curvularia lunata, Cylindrocladium scoparium, and investigated for their cytotoxic activities against SF-268, MCF-7, NCI-H460 and HepG-2 tumor cell lines by the SRB method. The results showed the extracts of 11 strains presented higher than 50%inhibitory rates against at least one of those plant pathogenic fungi at a concentration of 50 mg/mL and the extracts of 9 strains presented higher than 80%inhibitory rates against at least one of those tumor cell lines at a concentration of 100μg/mL. Among all strains studied, Eupenicillium sp. FS100, Penicillium sp. FS105, Dichotomomyces cejpii FS110 and Acaromyces ingoldii FS121 exhibited obvious inhibitory activities against plant pathogenic fungi and/or tumor cell lines.