For understanding the microbial community structure of soil rhizosphere with two typical diseases and clarifying the correlation between soil diseases and microbial diversity,high-throughput sequencing was applied to compare and analyze the microbial diversities of the rhizosphere with 2 typical diseases. As results,the population diversity and enrichment of bacteria in soil sample were the scab disease > CK> root-rot disease. While in fungi,the changes were more obvious,fungal diversity and enrichment in the soil with 2 diseases were higher than the control group. The species annotation showed that comparing with the control group,the bacterial species of different treatments were almost the same,but just the proportion of each were not identical. There were some specific fungal species which may be related to disease occurrence,and variation of proportion was significant,indicating that the changes of soil microbial structure affect the happening of the disease in a certain extent.%为了解两种典型病害土壤根际微生物群落结构,明确土壤病害与微生物多样性变化的关系,采用高通量测序对两种典型病害根际微生物多样性进行对比分析,结果表明,细菌多样性和丰度值表现为糊头病的土壤>对照组>根茎腐病组;而真菌变化更明显,两种病害土壤真菌多样性和丰富度均高于对照组.物种分类注释表明,不同处理土壤细菌种类与对照组基本一致,只是不同类别所占的比例各不相同,真菌中含有一些与病害发生有关的特有属,且比例变化较大,说明土壤微生物群落结构变化在一定程度上影响病害的发生.
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