基于已有研究工作基础,从蒙古沙冬青[Ammopiptanthus mongolicus(Maxim)Cheng.f]中分离到1个编码MYB类转录因子基因,命名为AmMYB4-like.该序列长度为1145 bp,含有一个由960 bp组成的开放阅读框,编码319个氨基酸,具有R2和R3保守结构域,属于R2R3-MYB转录因子.荧光定量PCR分析结果表明,AmMYB4-like在叶片中参与低温和干旱胁迫应答,在根中主要参与干旱胁迫应答.构建了pPZP212-AmMYB4-like植物表达载体,旨为进一步研究AmMYB4-like的功能奠定基础.%Based on our previous work,a gene encoding MYB transcription factor was cloned from Ammopiptanthus mongolicus,and it was designated as AmMYB4-like. The full length of the AmMYB4-like cDNA was 1,145 bp,including a 960 bp opening reading frame(ORF) which encoded a 319-amino acid peptide. AmMYB4-like contained R2 and R3 conserved domains and belonged to the R2R3-MYB subfamily. Quantitative real-time PCR(qRT-PCR)analyses revealed that AmMYB4-like was up-regulated in leaves under low temperature and drought stresses,but it was mainly induced by drought stress in roots. Plant expression vector pPZP212- AmMYB4-like was constructed for further research of AmMYB4-like.
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