多元复合酶降解黄姜薯蓣皂苷元的工艺研究

摘要

研究了多元复合酶降解黄姜薯蓣皂苷工艺条件对黄姜薯蓣皂苷元收率的影响因素。结果表明：采用碱性果胶酶＋单宁酶＋半纤维素酶＋β-葡萄糖苷酶4元复合酶的组合形式，在酶用量为黄姜量的1．5％，pH值为6．5，料液比为1∶4（g∶g），50℃下酶解10 h，以乙醇为萃取剂的条件下进行的降解效果最好，薯蓣皂苷元的收率最高可达4．34％。相比收率仅为1．61％的传统的酸解工艺，应用多元复合酶解工艺最高可使黄姜薯蓣皂苷元的收率提高1．69倍。%This paper focused on the impact factors of enzymatic hydrolysis by applying multivariate composite enzyme in the process.The results indicated that the quaternary multivariate composite enzyme, i.e.alkaline pectinase +tannase +hemicellulase+β-glycosidase,had the best degradation effect.The highest yield of diosgenin was 4.34 %,when the condition was 1.5 % compound enzyme of turmeric under 50 ℃,pH value 6.5,solid-liquid ratio of 1∶4 and ethanol as extraction solvent for 10 h.Compared with the traditional acid hydrolysis which yield was only 1.61 %,application of multivariate composite enzyme hydrolysis can improve the highest yield of diosgenin to 1 .69 times.