Objective We use recombinant Ad-ING4 and Ad-PTEN to infect human hepatocarcinoma cell line SMMC-7721 in vitro to test and compare the effects of gene expression of the cells. Methods We use recombinant Ad-ING4 and Ad-PTEN and control virus to infect QBI-293A cells for viral propagation and viral titration. The infective effects of Ad-ING4, Ad-PTEN and control virus were confirmed by fluorescence microscopy. Use Ad-ING4 and Ad-PTEN to infect SMMC-7721 cells and confirm their effects by their apoptotic rate and the results of MTT.Results The apoptotic rate of control virus group is 3.4% ± 1.3%, the Ad-ING4 group is 49. 7% ±4. 8% (P < 0. 01 )and the Ad-PTEN group is 11.4% ± 3.2% (P < 0. 01 ). The cell proliferation of group Ad-ING4 and AdPTEN obviously decreased from 48h to 96h( P <0.05 ). Conclusion The data analysis showed Ad-ING4 and AdPTEN had an inhibitory effect to SMMC-7721 cells especially that of Ad-ING4.%目的 用构建好的重组腺病毒Ad-ING4和Ad-PTEN感染人肝癌SMMC-7721细胞,检验其对细胞的作用.方法 用构建好的重组腺病毒Ad-ING4和Ad-PTEN感染QBI-293A细胞,对病毒进行扩增并测定其效价,荧光显微镜下观察感染效果.用扩增得到的Ad-ING4和Ad-PTEN感染SMMC-7721细胞,流式细胞术及MTT检测作用效果.结果 空腺病毒组的凋亡率为3.4%±1.3%,Ad-ING4组和Ad-PTEN组分别为49.7%±4.8%和11.4%4±3.2%,均显著高于空腺病毒组(P<0.01);Ad-ING4组和Ad-PTEN组的细胞增殖在48~96 h时较正常组明显下降(P<0.05).结论 Ad-ING4和Ad-PTEN对SMMC-7721细胞均有显著抑制作用,尤其是Ad-ING4.
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