敲低错配修复基因hMLH1可导致人胚肾细胞株293t的微卫星不稳定

摘要

Objective To explore the ralations between expression of hMLHl gene and microsatillites stability in human embryonic kidney cell line 293t. Methods A plasmid vector encoding shRNA targeting hMLHl (pGPU6/GFP/ Neo-shMLHl) was constructed for knocking down hMLHl gene in 293t cell line,and evaluated the microsatallite stability before and after knocking down. Results The expression of MLH1 in knocking down group was obviously knocked down in 293t cell, and the microsatallite states was changed from microsatallite stability into microsatallite instability. Conclusions Knocking down hMLHl gene may lead to microsatallite instability in cell line 293t.%目的 探讨人胚胎肾细胞株293t中错配修复基因hMLH1的表达量与微卫星稳定性的关系.方法 构建针对hMLH1基因的shRNA表达载体,并干扰293t细胞hMLH1的表达,评价敲低hMLH1前后293t细胞微卫星状态的变化.结果 干扰组293t细胞相比对照组hMLH1表达明显敲低,微卫星状态由稳定变为不稳定.结论 敲低hMLH1可导致293t细胞的微卫星不稳定.