Phytochemical composition andin vitro antioxidant activity of aqueous extract ofAerva lanata(L.) Juss. ex Schult. Stem (Amaranthaceae)

摘要

Objective:To analyze the phytochemical composition andin vitro antioxidant properties of aqueous extract ofAerva lanata(A. lanata) stem.Methods:During the preliminary phytochemical analysis, the aqueous extract ofA. lanata was screened for the presence of carbohydrates, proteins, phenolic compounds, oil and fats, saponins, flavonoids, alkaloids, tannins and phytosterols.Antioxidant activity of the extract was determined by2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, metal chelating activity, reducing power activity andDNA damage inhibition activity.Analysis of phenolic compounds was performed byFolin-Ciocalteau reagent method and gradient high performance liquid chromatography technique. Results:Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins, flavonoids, tannins and phytosterols as major phytochemical groups.The extract exhibited high2,2-diphenyl-1-picrylhydrazyl radical scavenging activity(IC50=110.74 μg/mL), metal chelating activity(IC50=758.17 μg/mL), reducing power activity andDNA damage inhibition efficiency.The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid(3,4,5-OH), apigenin-7-O-glucoside(apigetrin), quercetin-3-O-rutinoside(rutin) and myricetin(3,5,7,3,4,5-OH) by high performance liquid chromatography analysis.The extract was found non toxic towards human erythrocytes in the hemolytic assay(IC50=24.89 mg/mL).Conclusions:These results conclud thatA. lanata stem possesses high antioxidant activity and can be used for the development of natural and safe antioxidant compounds.