Expressmn Specificity of Hepatic IGF-1 mRNA and Its Correlation with Body Weight During Early Development of Ducks

摘要

Gaoyou and Jinding ducks with different growth rates were selected to conduct the test,and a comparative study was carried out on the expression pattern of liver insulin-like growth factor 1( IGF-1) mRNA and its correlation with body weight in the early embryonic stage and hatching stage. The expression pattern of hepatic IGF-1mRNA of ducks at embryonic ages of 13,17,21,25,27 and 0- 7 days after hatching were monitored by real-time fluorescent quantitative PCR. The results showed that expression of duck hepatic IGF-1 mRNA could be detected at the embryonic age of 13. Body weight and hepatic IGF-1 mRNA expression of ducks both showed an increasing trend during the early development. The expression of hepatic IGF-1 mRNA of Gaoyou ducks at embryonic ages of 13,21 and on the 7thday after hatching was extremely significantly higher than that of Jinding ducks( P < 0. 01),which was consistent with the changes of daily weight gain and absolute growth rate. The changes of daily weight gain and hepatic IGF-1 mRNA expression both showed extremely significant specificity of the breeds and time during the entire observation( P < 0. 01),and which was extremely significantly affected by the interaction of the breeds and time( P < 0. 01). The analysis on breeds and gender manifested that the change of body weight was consistent with that of hepatic IGF-1 mRNA expression,which showed an extremely significant positive correlation( P < 0. 01). The results indicated that hepatic IGF-1 mRNA expression played an important role during the early development of ducks.