Differential Expression of CCNA2 Gene and MMP2 Gene in Skin Tissue of Rex Rabbit with Different Fur Densities

摘要

[Objective] The paper was to search for the molecular marker highly associated with fur density of rex rabbit.[Method] Experimental rex rabbits were divided into dense fur group and sparse fur group.The variation rule of gene expression in skin tissue of rex rabbits with different fur densities was detected using rabbit whole gene c DNA microarray.[Result] A total of 2 657 genes with differential expression were detected by microarray test,including 1 106 genes with known function,687 genes with up-regulated expression and 419 genes with down-regulated expression.The expression levels of multiple genes involving in cell proliferation,differentiation,apoptosis and migration had significant changes.MMP2 gene had high expression in skin tissue of rex rabbits in dense fur group,which was 8.195 times of gene expression in sparse fur group; CCNA2 gene had low expression in skin tissue of rex rabbit in dense fur group,which was0.143 times of gene expression in sparse fur group.[Conclusion] The results provide the theoretical basis and research direction for early breeding of rex rabbit in practical production.