小花棘豆对小白鼠的毒性试验

摘要

60 Kunming mice were randomly divided into 5 test groups （A, B, C, D, E）with different doses of Oxytropis glabra DC （60%, 40% ,20%, 10% ,5% ）and a control group. The toxicity test of O. glabra was carried out for 60 days. The results showed that mice that were fed with the diet with adding 20% O. globra all survived within 60 days. And mice in the group of adding 40% O. glabra began to die after 57 days. And mice in the group of adding 60% O. glabra began to die after 45 days. The weight gain rate of mice obviously decreased with the increasing of O. glabra in the diet. The final weight of mice in A, B, C test groups was extremely significantly different with control group （P〈0.01 ）. And the weight gain of mice in D test group was significantly different with control group （P〈0.05） and that in E group had no significant difference with control group. Compared with control group ,the viscera coefficients （heart, spleen, brain and lung） of mice in different dose groups changed little. The liver coefficient and kidney coefficient increased with the increasing of the dose of O. glabra. The higher the content of O. glabra in the diet was, the more seriously mice＇s liver and kidney were swollen. And the differences of mice＇s liver and kidney between A, B, C groups and control group was extremely significant （P〈0.01）, which O. gla/bra would caused the injury to the liver and kidney when the dose of O. glabra reached 20%.%将60只昆明系小白鼠随机分为5个剂量的小花棘豆试验组（A、B、C、D、E组小花棘豆剂量分别为日粮的60％、40％、20％、10％、5％）和1个对照组（F组）．进行为期60d的小花棘豆毒性试验。结果表明，在小白鼠日粮中添加20％小花棘豆，在60d试验期内未出现小白鼠死亡；添加40％小花棘豆，第57天出现死亡；添加60％小花棘豆，第45天出现死亡。随着小花棘豆在日粮中含量的增加。小白鼠的增重幅度明显减慢，其中A、B、C组小白鼠的试验终体重与对照组差异极显著（P〈0．01），D组与对照组差异显著（P〈0．05），E组与对照组无显著性差异。各剂量组小白鼠与对照组小白鼠相比．心脏、脾脏、脑和肺脏的脏器系数变化不大；肝脏系数和肾脏系数随着小花棘豆剂量的增加而增大，日粮中小花棘豆含量越高，小白鼠肝、肾肿大越严重，A、B、C组与对照组相比较差异极显著（P〈0．01），表明日粮中小花棘豆剂量达到20％时．就会对肝、肾脏造成损伤。