17α-甲基睾酮对稀有鲫体内激素水平的影响

摘要

It is well known that endocrine disrupting chemicals (EDCs) is harmful for the health of animals and human. Most of the EDCs eventually enter into the water environment and cause damage of reproductive system and endocrine system of aquatic organisms. In the present study, the Gobiocypris rarus was used as material to evaluate the effect of MT exposure on its hormone levels. The concentrations of E2, T and 11-KT in adult female and male Gobiocypris rarus after exposure of MT for 7 days were determined by ELISA. The results revealed that compared to control groups, the concentrations of E2, T and 11-KT in female fish exposed in MT were significantly up-regulated (P<0.01);however, in male fish exposed in MT, the concentrations of E2, T and 11-KT were significantly down-regulated (P<0.01).%环境内分泌干扰物（EDCs）对动物和人的健康有一定的危害作用，很大一部分环境内分泌干扰物最终都进入到了水环境中，对水生生物的生殖、内分泌等系统造成损伤。以稀有鮈鲫作为毒性试验材料，选择具有代表性的雄激素17α-甲基睾酮（MT）作为研究对象，采用酶联免疫法（ELISA）研究MT处理7 d对稀有鮈鲫体内雌二醇（E2）、睾酮（T）和11-酮基睾酮（11-KT）含量的影响。结果显示，经MT处理7 d后，雌鱼体内的E2、T和11-KT含量均极显著高于对照组（P<0.01），而雄鱼体内的E2、T和11-KT含量均极显著低于对照组（P<0.01）。