Aim To establish a method of fingerprint analysis on Spica Prunellae by HPLC. Methods The HPLC method being used,chromatographic conditions were BIOSIL U546250-1 C18 (250 mm × 4. 6 mm , 5 μm) . Mobile phase was methyl alcohol-water-acetate-tri-ethylamine (84 ∶ 16 ∶ 0. 05 ∶ 0. 03) . The flow rate was 0. 8 ml · min -1. The detective wavelength was at 215 nm , and the column temperature was 30℃ . Results Establishing the fingerprint of Spica Prunellae , 12 common peaks were found in the HPLC fingerprint of Spica Prunellae and good similarities with correlation coefficients over 0. 87 were found in the fingerprints between the herbs from different sources and standard fingerprint ,which could be utilized for the identification of Spica Prunellae. Conclusion The method is accuate.simple, and could be used for the quality control of Spica Prunellae.%目的 建立夏枯草HPLC指纹图谱分析方法.方法 采用高效液相色谱法,BIOSIL U546250-1型色谱柱(4.6 mm×250 mm,5 μm),流动相:甲醇-水-醋酸-三乙胺(84:16:0.05:0.03),流速0.8 ml·min-1,柱温30℃;检测波长215 nm.结果 建立了夏枯草的指纹图谱,确定了12个共有峰,各夏枯草样品指纹图谱与对照指纹图谱相似度均在0.87以上,可用于夏枯草药材的定性鉴别.结论 该方法简单、准确、重现性好,为夏枯草的质量控制提供了有效的方法.
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